http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058215 Abstract Effects of C-phycocyanin (C-PC), the active component of Spirulina platensis water extract on the expressions of N-methyl D-aspartate receptor subunit 2B (NR2B), tumor necrosis factor–α (TNF-α), interleukin-1β (IL-1β), and cyclooxygenase type 2 (COX-2) genes in the cochlea and inferior colliculus (IC) of mice were evaluated after tinnitus was induced by intraperitoneal injection of salicylate. The results showed that 4-day salicylate treatment (unlike 4-day saline treatment) caused a significant increase in NR2B, TNF-α, and IL-1β mRNAs expression in the cochlea and IC. On the other hand, dietary supplementation with C-PC or Spirulina platensis water extract significantly reduced the salicylate-induced tinnitus and down-regulated the mRNAs expression of NR2B, TNF-α, IL-1β mRNAs, and COX-2 genes in the cochlea and IC of mice. The changes of protein expression levels were generally correlated with those of mRNAs expression levels in the IC for above genes. Although some medications appeared to be effective for tinnitus, the results were still not so satisfactory and/or their adverse side effects prevent them as a regular treatment for mice and/or humans –. Therefore, it is still worth searching for more “safe and effective” medications for tinnitus. Spirulina is a microscopic blue-green algae living both in sea and fresh water. It is composed of high quality protein, iron, gamma-linolenic fatty acid, carotenoids, vitamins B1 and B2, minerals, and its active component: C-phycocyanin(C-PC), etc . It has been reported that Spirulina platensis water extract or C-PC exerts anti-oxidative, anti-inflammatory activities and neuroprotective effects via inhibition of COX  and/or nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzymes . Meanwhile, as we mentioned above, tinnitus were associated with up-regulation of the NR2B and proinflammatory genes , . So, Spirulina might be also a good candidate for prevention or treatment of tinnitus. In this study, we aim to investigated whether C-PC or spirulina platensis water extract could reduce the tinnitus score and expression levels of NR2B, TNF-α, IL-1β, and COX-2 genes in the cochlea and IC in response to intraperitoneal injections of salicylate. Results This experimental study showed that the both of spirulina platensis water extract and its active component (C-PC) could reduce salicylate-induced tinnitus and reduce expression of NR2B,.... ..... In conclusion, we suggested that spirulina platensis water extract and its active component (C-PC) could reduce salicylate-induced tinnitus. The beneficial role of spirulina mainly via inhibiting expression of NR, COX-2 and proinflammatory genes.