Potent Anti-Seizure Effects of D-Leucine

Discussion in 'Research News' started by Danny Boy, Feb 5, 2016.

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    1. Danny Boy
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      Danny Boy Member Benefactor Hall of Fame

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      "Even at low doses, D-leucine ( amino acid) suppressed ongoing seizures at least as effectively as diazepam but without sedative effects. These studies raise the possibility that D-leucine may represent a new class of anti-seizure agents, and that D-leucine may have a previously unknown function in eukaryotes."

      "In an initial set of experiments, researchers pre-treated mice with the amino acid L-leucine and another one, called D-leucine, which has a nearly identical structure to L-leucine and is essentially its biochemical mirror image.

      When researchers induced seizures with shock therapy, animals pre-treated with either amino acid fared better, developing seizures at notably higher electric currents than mice that received placebo, a sign of greater seizure resistance.

      To see whether D-leucine and L-leucine could also interrupt ongoing seizures, researchers induced seizures in a group of animals and, once convulsions began, they administered low and high doses of both amino acids. L-leucine failed to abort ongoing seizures, while D-leucine effectively interrupted convulsions. Strikingly, the researchers say, D-leucine terminated seizures even at low doses. Next, researchers compared the ability of D-leucine to terminate prolonged, unrelenting seizures against the sedative diazepam, commonly used stop such seizures in humans. Both treatments terminated seizures, but D-leucine did so about 15 minutes earlier. In addition, mice treated with D-leucine resumed normal behavior faster and experienced none of the drowsiness and sluggishness observed in animals treated with the drug, also common side effects seen in human patients.

      A final set of experiments measured D-leucine interaction with several nerve receptors known to be involved in cell-to-cell signaling and seizure activity. Surprisingly, D-leucine interacted with none of the signaling pathways known to spark or avert seizures.

      “Our results suggest that D-leucine affects neurons differently from other known therapies to control seizures,” says senior investigator J. Marie Hardwick, Ph.D., the David Bodian Professor in microbiology and immunology at the Johns Hopkins Bloomberg School of Public Health. “This finding gives us hope of new approaches to epilepsy on the horizon.”

      Both L-leucine and D-leucine protect mice against seizures.


      http://www.ncbi.nlm.nih.gov/pubmed/26054437

      http://www.hopkinsmedicine.org/news...entify_amino_acid_that_stops_seizures_in_mice

      http://www.amazon.com/BulkSupplements-Pure-L-Leucine-Powder-grams/dp/B00GW5ZHI2/

      http://www.amazon.co.uk/gp/product/B00HF61IB2/
       
      • Informative Informative x 2
    2. ady

      ady Member

      @Danny Boy what is your affection toward anti seizure medications? It's not been proven they help tinnitus at all!
       
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    3. Danny Boy
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      Danny Boy Member Benefactor Hall of Fame

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      It's not a medication, it's an amino acid. Also Clonazepam an anti-seizure med, helps with tinnitus. And this is a far better alternative, is it not?

      Also this part-

      “Our results suggest that D-leucine affects neurons differently from other known therapies to control seizures,” says senior investigator J. Marie Hardwick, Ph.D., the David Bodian Professor in microbiology and immunology at the Johns Hopkins Bloomberg School of Public Health. “This finding gives us hope of new approaches to epilepsy on the horizon.”
       
    4. Santiago

      Santiago Member

      Tinnitus Since:
      November 2012
      Cause of Tinnitus:
      Loud Sound Exposure, Acoustric Trauma
      Interesting @Danny Boy , so this a thing that can be easy obtained?, Have you been taking it ?
       
    5. Danny Boy
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      Danny Boy Member Benefactor Hall of Fame

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      It is easily absorbed. I'm getting some today, via amazon same day delivery. Worth a try.
       
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    6. Santiago

      Santiago Member

      Tinnitus Since:
      November 2012
      Cause of Tinnitus:
      Loud Sound Exposure, Acoustric Trauma
      So I believe there's difference between D-Leucine and L-Leucine, and it must be specially D-Leucine in this case?
       
    7. AnxiousJon
      Magical

      AnxiousJon Member

      Location:
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      Looks like D is not sold for human consumption; only lab testing. L still had some results on the mice. I supose this could help people with myoclonus if it can be absorbed effectively enough when ingested--though it looks like D is the real deal.
       
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    8. Danny Boy
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      Danny Boy Member Benefactor Hall of Fame

      Location:
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      Both L-leucine and D-leucine protect against seizures.

      "Regular leucine is technically "D-leucine" signifying the chirality of the compound.

      According to the abstract of this research paper, (http://dmd.aspetjournals.org/cgi/con...act/30/12/1436) D-leucine can be converted into l-leucine in the body in a 2 step reaction. INTERESTING POINT. If you go on to read further into the paper they say that originally it was thought d-amino acids were not used by mammals but more recent studies (circa 2000) suggest that they are in fact used.

      The paper goes on to reference a study about nitrogren balance:

      "Use of D-amino acids for the purpose of growth or maintenance of nitrogen equilibrium was repeatedly confirmed by different species of animals for about 40 years, and it was found that several D-amino acids were used for growth (Meister, 1965; Friedman, 1999). In these studies, a widely accepted method involves a comparison of growth rate of animals fed a control diet with that of animals fed a diet containing the D-amino acid instead of the corresponding L-enantiomer. The fraction that converted from D-amino acid to the L-enantiomer was estimated from the dosage of the D-amino acid required to achieve the same growth rate in control animal. However, the technique is very laborious and cannot readily be applied to human subjects. "

      In the study of the paper, researchers looked at this process and determined that when injected intravenously approximately 30% of d-leucine was converted into l-leucine. No l-leucine was converted into d-leucine."

      http://www.amazon.com/BulkSupplements-Pure-L-Leucine-Powder-grams/dp/B00GW5ZHI2/

      http://www.amazon.co.uk/Protein-Dynamix-Micronized-Leucine-amino/dp/B011IS7T8S/
       
      • Helpful Helpful x 1
    9. PatrickG

      PatrickG Member Benefactor

      Location:
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    10. Danny Boy
      Cheerful

      Danny Boy Member Benefactor Hall of Fame

      Location:
      England
      Tinnitus Since:
      7/2014
      Cause of Tinnitus:
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      You can also get the L version from the same website. D-leucine is normal leucine. I've found normal leucine here-

      http://www.amazon.com/BulkSupplements-Pure-L-Leucine-Powder-grams/dp/B00GW5ZHI2/

      http://www.amazon.co.uk/Protein-Dynamix-Micronized-Leucine-amino/dp/B011IS7T8S/


      http://www.sigmaaldrich.com/catalog/substance/lleucine131176190511?lang=en&region=GB


      "We investigated the two exclusively ketogenic amino acids, L-leucine and L-lysine, and found that only L-leucine potently protects mice when administered prior to the onset of seizures induced by kainic acid injection, but not by inducing ketosis. Unexpectedly, the D-enantiomer of leucine, which is found in trace amounts in the brain, worked as well or better than L-leucine against both kainic acid and 6Hz electroshock-induced seizures. However, unlike L-leucine, D-leucine potently terminated seizures even after the onset of seizure activity. Furthermore, D-leucine, but not L-leucine, reduced long-term potentiation but had no effect on basal synaptic transmission in vitro. In a screen of candidate neuronal receptors, D-leucine failed to compete for binding by cognate ligands, potentially suggesting a novel target. Even at low doses, D-leucine suppressed ongoing seizures at least as effectively as diazepam but without sedative effects"



      But both can be used for seizure management.
       
    11. Jahhsoul
      Curious

      Jahhsoul Member

      Location:
      Spain
      Tinnitus Since:
      9/24/2015
      Cause of Tinnitus:
      Unkown (not acute acoustic trauma).
      We cannot buy in sigmaaldrich, they only sell for investigation purposes. I tried it with D-met.
       
    12. Cheza
      Wishful

      Cheza Member Benefactor

      Location:
      Oregon
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      04/2014
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      I thought you had your tinnitus well under control with Trobalt, even after ceasing to use it. Are you planning to use the leucine as a preventive measure, or is your tinnitus at a level where you need to subdue it again?

      For U.S. members, here's the short link to a highly-rated leucine product on Amazon.com: http://www.amazon.com/BulkSupplements-Pure-L-Leucine-Powder-grams/dp/B00GW5ZHI2/

      Edit to add, the manufacturers state in the Q&A that this leucine is derived from either duck feathers or lecithin, depending on the batch they have in stock. This is not an acceptable product for vegans and vegetarians.


      20fcNuR.jpg
       
    13. Jahhsoul
      Curious

      Jahhsoul Member

      Location:
      Spain
      Tinnitus Since:
      9/24/2015
      Cause of Tinnitus:
      Unkown (not acute acoustic trauma).
      That´s L not D-Leucine.
       

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