Celastrol Enhances Atoh1 Expression in Inner Ear Stem Cells and Promotes Their Differentiation

https://www.researchgate.net/figure...ownstream-signaling-A-The-mouse_fig5_41421769

Regulation of HES1 by TGFB1

And I was wrong, it is a good question.
Medical celastrol real prices:

https://www.sigmaaldrich.com/catalog/product/sigma/c0869?lang=pl&region=PL
https://www.trc-canada.com/product-detail/?C249500
http://www.aktinchem.com/product/18/celastrol-tripterine-437.html?
gclid=Cj0KCQjw9ZDeBRD9ARIsAMbAmoaoXoHXSNlN90npu3IXhpfu6Iw3OXhJpwwq__q9x_CowZU3xcmmjHMaArsLEALw_wcB

 

Celastrol is a strong anticancer.

 

I think there is about 50mg/kg Celastrol in extract of plant or even less. So not too much. From 17kg of roots was extracted 46.8g extract1 and 37g extract2.

Extract1- 0.09% had celastrol content
Extract2- 2.4 % had celastrol content.
(By hplc)

But here:
https://www.globalsources.com/si/AS...erygium-Wilfordii-Hook-Extract/1149876352.htm

They write also 98% measured by hplc.
Can it be the truth?

 

Its strong expensive.

 

How do we get msx1 and msx2?

 

Not so expensive from China
https://globalsources.com/si/AS/Her...erygium-Wilfordii-Hook-Extract/1149876352.htm

https://www.researchgate.net/public...c_Approach_to_Reveal_Specific_Gene_Activation

Look at table. 1
They are in a herbal mix of astragalus and angelica.

Moreover it contains many other helpful things.

 

What chances do you think this has to actually cure our tinnitus?

 

Much higher than regenerative hearing.
Astragalus is very powerful.

Last time I thought much about thru-bone distribution of medicine. However Its very deep in skull.

 

But isn't the goal here to regenerate out hearing with this stuff by causing expression of atoh1 which regrows hair cells?

I just received the thunder god supplement and this deer antler spray. I almost feel silly trying to actually heal myself.

 

You know. We would be first in history who made this. Besides I'm tired of this shit anyway. I would not like to have to be interested in this at all.

 

You'd think there would be biotech firms all over the world racing to develop a treatment. Right now. Nope.

 

"For inner ear treatment, local application of IGF-1 is a more preferable drug delivery method compared with systemic delivery, because blood flow of inner ears is only 1/10000 to 1/1000000th of the systemic flow."

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159992/#__sec5title

 

So what? Injections?

 

Yes, this is probably our only hope. This will take years unless a miracle occurs. My tinnitus has gotten significantly worse over the last week and a half as well from noise exposure. I'm really scared for my future, and my family because this will surely affect them as well.

 

I face a similar fear about my future. I've really enjoyed reading your post with PolishSoldier as you are both thinking outside the box. I don't want to hijack this thread but have you tried Nicotinamide Riboside?

https://www.tinnitustalk.com/thread...lly-prevents-noise-induced-hearing-loss.7101/

 

At least someone is reading this thread so its not that bad. I fear about complications with this treatment.

"This function requires at a minimum that IHCs lose their kinocilia in order for their stereocilia to move freely from the inner spiral sulcus to the subtectorial space, and back. To achieve this, IHCs have thick stereocilia and are immediately adjacent to each other to maximally obstruct the fluid flow from the sub-tectorial space to the inner spiral sulcus. Since the reticular lamina is only displaced by approximately 2 nm at 70 dB sound pressure level, it is essential to have this maximal obstruction of fluid flow so that limited movements of endolymph at the tallest stereocilia causes enough displacement to stimulate IHCs. Embedding a kinocilium into the overlying tectorial membrane and reshaping IHC stereocilia like vHC bundles would hamper this IHC function. In contrast, presence of a kinocilium and embedding it into the tectorial membrane is fully compatible with basilar papilla mediated sound sensing in other vertebrates.
Good artice:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092489/
Look at pictures. Explain many things.

Problem can be wrong shape of Ihc and lenght of cilias and distance from their roof.

 

very good work man. what's up with medical regulations in Poland? can we get an ENT in Poland to inject our ears with EGF + 7,8 DHF, + Fosamax?

 

Officially not but anywhere yes where blackmarket exists. Would you try?

Frequency have their own way to transport medicine to inner ear.
Other cases it would be washed out and not reach cochlea, i think.
It would be too easy other way. Intratymphanic injections are not any novelty. That's why im trying to find way to orally modulate the genes.

 

hydrogel and or bisphosphonate my friend.

 

What proportion? What form? I don't know. It's extremely risky. Need own lab and crew to do this. And volunteers.

 

we need to scheme on finding an ENT to just inject our ear drums with 7,8 dhf, bisphosphonate, and EGF. it is a big world, fuck America and the EU.

 

Even if this worked it only would repair some synapses in damaged hair cells that have not fully lost their connections
with nerves and luckily survived.

Dead hair cells would remain dead.

 

this report says the hair cells regrew.
The researchers found that activating the ERBB2 pathway triggered a cascading series of cellular events by which cochlear support cells began to proliferate and start the process of activating other neighboring stem cells to become new sensory hair cells. Furthermore, it appears that this process not only could impact the regeneration of sensory hair cells, but also support their integration with nerve cells.

https://www.sciencedaily.com/releases/2018/10/181015132953.htm

 

They don't know what has grown, how much or in what layout. Some assumptions.

 

I took thunder god and igf1 for 2 days and it seems to spike my tinnitus badly

 

Not good. Are you going to stop?

 

yup, going back on high doses of curcumin. that really seemed to help more so than anything. I can't handle day long super spikes. then again im not ever sure if it is the supplements or the noise ive been exposed to. this is a confusing nightmare/

 

Is disappearance of tinnitus and hearing complete silence (complete silence, even my reactive tinnitus from ultra high frequency hearing loss in right ear) normal thing? I woke up and had some hum but I slept for some hours more, wake up and it was normal. Like before. Zero.

Last days are the best. It is quieter and less reactive everyday.

Is it normal behavior for tinnitus that it is being silenced in silence?

I have neuro-lyme.
I have many more strange symptoms (shaking vision) but my vestibulo-ocular reflex and optocinetic responses are good (wtf1) I lost some of my hearing after amoxycillin (wtf2) and get green toxin stains on face.

What do you think about it.
Can be this all neurolyme related?

 

Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 (...)
https://www.hindawi.com/journals/ecam/2014/965486/

 

I wish we were super rich and could fly back and forth to America and Poland and set up labs like Neill Breen/Batman and figure this out on our own. I really think we could make rapid progress. We are totally assuming HC regeneration would solve our issues. Plus there is a consideration of systemic vs local gene manipulation which may be the entire impasse.