Lu Rong (Deer Antler)

Anyone tried this supplement?

https://translate.googleusercontent...700214&usg=ALkJrhh4FDDugwLy8nvGMi9qfzhZRqBmow

In the article, there are 40 interesting links to PubMed, such as these:

[Protective effect of velvet antler polypeptide (VAP) on rats with the spinal cord injury].

Abstract
OBJECTIVE:
To investigate the protective effect of antler polypeptide on the rats with spinal cord injury (SCI).

METHODS:
The model rats were treated with different doses of antler polypeptide, and its effect on motor function, ethology and pathological changes of spinal cord of the rats observed.

RESULTS:
Seven days after treatment with different doses of antler polypeptide, rat's motor activity was recovered in some extent. Significant difference (P < 0.001)was found between the antler polypeptide treatment group and operation group. The effect could be enhanced by increase of the doses. We observerd the effect on the pathological change of spinal cord in rat, and found the tissue edema and inflammatory infiltration were relieved after treatment with different doses of antler polypeptide, especially in the dose of 15 mg antler polypeptide.

CONCLUSION:
Antler polypeptide can promote the motor function recovery in SCI rats, and its action is dose-dependent.


Biological effect of velvet antler polypeptides on neural stem cells from embryonic rat brain.

Abstract
BACKGROUND:
Velvet antler polypeptides (VAPs), which are derived from the antler velvets, have been reported to maintain survival and promote growth and differentiation of neural cells and, especially the development of neural tissues. This study was designed to explore the influence of VAPs on neural stem cells in vitro derived from embryonic rat brain.

METHODS:
Neural stem cells derived from E12-14 rat brain were isolated, cultured, and expanded for 7 days until neural stem cell aggregations and neurospheres were generated. The neurospheres were cultured under the condition of different concentration of VAPs followed by immunocytochemistry to detect the differentiation of neural stem cells.

RESULTS:
VAPs could remarkably promote differentiation of neural stem cells and most neural stem cells were induced to differentiate towards the direction of neurons under certain concentration of VAPs.

CONCLUSION:
Neural stem cells can be successfully induced into neurons by VAPs in vitro, which could provide a basis for regeneration of the nervous system.

Efect of velvet antler polypeptide on peripheral nerve regeneration].

Abstract
OBJECTIVE:
To investigate the velvet antler polypeptide (VAP) on sciatic nerve regeneration in rats through local administration and VAP-PLGA compound membrane.

METHODS:
The 3, 15 mg/g of VAP-PLGA compound membrane were prepared by compounding VAP and PLGA, respectively. Seventy-two Wistar rats, male or female, aged 3-6 months and weighing (250 +/- 50) g, were selected to make the model of sciatic nerve section. Then, all rats were randomized into 4 groups (n = 18): group A in which nothing was given after anastomosis, group B in which 1 mL of VAP at the concentration of 10 mg/L was injected into the gastrocnemius muscle medial for every other day, group C in which 3 mg/g of VAP-PLGA compound membrane was given to the nerve anastomotic stoma and group D in which 15 mg/g of VAP-PLGA compound membrane was given to the nerve anastomotic stoma. The sciatic adhesion degree observation, electrophysiological examination, immunohistochemical staining and hemi-quantity calculation and horseradish peroxidase (HRP) retrograde tracing were conducted 2, 4 and 6 weeks after operation, respectively.

RESULTS:
All rats survived to the end of the experiment, without foot ulcer or neuroma. Severer nervous adherence was observed in group A, mild adherence in group B, and no adherence in groups C and D 2, 4 and 6 weeks after operation, respectively. The recovery rate of the evoked potential of triceps surae in groups B, C and D was better than that in group A (P < 0.01), group D was superior to groups B and C (P < 0.05) at each time point. No significant difference between group B and group C (P > 0.05) 2, 4 weeks after operation was detected, but group C was superior to group B (P < 0.05) 6 weeks after operation. For the regenerative fiber axon and the expression of myelin sheath TGF-P1 and IGF antigen, the staining intensity in groups B, C and D was higher than that in group A at each time point (P < 0.05), and there were significant differences between group D and groups B and C 6 weeks after operation (P < 0.05), but no difference between groups B and C (P > 0.05). The HRP retrograde tracing showed that the myelinated nerve fiber stained by HRP gradually increased as time passed by and myelinated nerve fiber stained by HRP in groups B, C and D was much more than that in group A, and group D was superior to the other groups. No significant difference between group B and group C was detected.

CONCLUSION:
To apply VAP through either local administration or VAP-PLGA compound membrane around the attached site of nerve anastomosis is capable of promoting nerve regeneration, which has an obvious dose-effect relationship with the dose of VAP. Meanwhile, VAP-PLGA compound membrane can prevent the nerve adhesion.

 

Researchers from Wroclaw were the first in the world to grow industrial stem cells from deer antlers and proved that they give rise to all elements of which antler consists - cartilage, nerves, skin, bones, blood vessels.
The first breeding, named MIC-1, was created six years ago and since then it has been constantly expanded. Antler cells have extraordinary properties because they can divide very quickly and can differentiate into all types of tissues - epithelial, connective, muscular and nervous.
The wonderful properties of cells have already been observed on the example of mice, rabbits and sport horses. Scientists agree that it is one of the most regenerating preparations of natural origin. What's more, it can be used in both animals and humans.
Researchers from Wroclaw plan to use their discovery in regenerative medicine, veterinary medicine and cosmetology. Soon creams for mature skin with a strong regenerating effect, as well as preparations that accelerate hair growth, are to appear on the Polish market. Work is under way on drugs to help with inflammation, repair damaged bone tissue, treat wounds, ulcers and atopic dermatitis.


http://wyborcza.pl/1,75400,10785574,Leki_z_rogow_jelenia.html


- I read once that the horns that appear in a deer in the spring, grow at a phenomenal rate of two centimeters a day. The horns are warm, because such fast processes take place there; is the fastest growing tissue in animals. I was intrigued, I decided to examine the properties of their cells - says dr hab. Marek Cegielski from the Medical Academy in Wrocław, veterinary surgeon.

The advantages of deer specs have been known for a long time. In the world, mainly in New Zealand, Asia and the Americas, there are special farms only to obtain horns from which, among others, dietary supplements, anti-inflammatory properties, strengthening agents. - At the Olympic Games in Sydney, Russian players swallowed a pantocrine, i.e. an extract from an antler - says Dr. Cegielski.

He is the author of the discovery that in order to enjoy the benefits of antlers, it is not necessary to cut off further horns to individuals of Cervus elaphus (deer), but only to grow stem cells from them.

- It is the stem cells in the corners that give rise to all the elements from which the antlers are built: cartilage, nerves, skin, bones, blood vessels. The first in the world we proved that the cells that build the new antler are stem cells and we first bred them on an industrial scale - says the scientist.

He brought the first cell culture out of the disc, which he cut out to the deer at the Wrocław ZOO together with Ireneusz Całkosiński, also a veterinarian. The breeding line was called MIC-1: M for Marek, I for Ireneusz, C for Calkosiński and Cegielski, and 1 for Dr. Elzbieta Gębarowska, the only woman in the band.

Since then, breeding has been multiplied: cells are "immortal", from one sample from six years ago, more billions are added each week. They can be frozen and stored for years, after thawing they are still active. It turned out that the anticancer cells have extraordinary potential; they divide instantly and can differentiate into all types of tissues: epithelial, connective, muscular and nervous.

The cells from the antler transplanted to mice and rabbits regenerated damaged ear cartilage, bone of the jaw; the wounds healed much faster. Dr Cegielski admits that the repair effects of tendons in horse sports are "delightful" that it is one of the most regenerating preparations of natural origin that can be used in humans and animals.

- These cells seem to have no limitations: they regenerate the cartilage, which is not reborn in normal conditions, and bones and skin - says Dr. Wojciech Dziewiszek from the Department and Pharmacology Department of the Medical Academy in Wrocław.

The inhabitants of Wrocław created the Stem Cells Spin company and plan that their ideas will be used in regenerative medicine, veterinary medicine and cosmetology, in the form of creams, ointments, injections and oral medications. In the coming months, all over Poland, you can buy creams for mature skin with a strong regenerating effect and preparations stimulating hair growth. Their registration and preparation of the first samples are already underway. - Cosmetic preparations usually only fill in gaps in the skin, while the uniqueness of these lies in the fact that they strongly stimulate our own cells responsible for the synthesis of collagen and elastin, which are the "scaffolding" of the skin - say Dr. Danuta Nowicka, dermatologist at the Medical Academy.

Drugs to be created in subsequent years will be used, among others in the treatment of inflammation, repair of damaged bone tissue, in the treatment of wounds, ulcers and atopic dermatitis.

 

Cheap. 25 USD - 60 Tabs

https://podkowa.eu/pl/p/-Lu-Rong-Ekstrakt-z-rogu-jelenia-tabletki-500mg-/1691

Ordered already.

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159992/

CLINICAL TRIAL OF IGF-1 FOR SSHL
Based on these in vivo and in vitro studies, a clinical trial was performed to study the efficacy of IGF-1 in the treatment of SNHL (University Hospital Medical Information Network Clinical Trials Registry under trial registration number UMIN000000936; Nakagawa et al., 2010, 2012). In this clinical trial, 25 patients with SSHL that was refractory to systemic steroid therapy were recruited and IGF-1 was applied onto the round window membrane, using gelatin hydrogel, as performed in in vivo animal studies (Iwai et al., 2006; Lee et al., 2007; Fujiwara et al., 2008). The outcome of IGF-1 treatment was compared with that of a historical control after hyperbaric oxygen therapy where 66 out of 199 patients (33%) showed the hearing threshold improvement (Nakagawa et al., 2010). In this trial, improvement of hearing threshold was defined as recovery of more than 10 dB in the mean hearing level at the five frequencies tested (0.25, 0.5, 1.0, 2.0, and 4.0 kHz). At 12 and 24 weeks after IGF-1 treatment, the proportions of patients showing hearing improvement was 48 and 56%, respectively. No serious adverse effects were observed. The proportion of hearing improvement at 24 weeks after IGF-1 treatment was significantly better than that of the historical control. Analyses of recovery of threshold in each frequency tested revealed that the thresholds for lower frequencies recovered better than those for higher frequencies (Nakagawa et al., 2012). Average recovery in pure tone audiometry thresholds over the five frequencies tested was 11.9 dB (Nakagawa et al., 2012), which was comparable to the average threshold recovery previously reported for intra-tympanic steroid injection (5–22 dB). A randomized clinical trial, comparing the efficacy of topical IGF-1 treatment with that of intra-tympanic steroids for SSHL that is refractory to systemic steroids, is now underway, involving about 120 patients from all over Japan (University Hospital Medical Information Network Clinical Trials Registry under trial registration number UMIN000004366).

CONCLUSION
In conclusion, IGF-1 is a promising medication for SNHL. IGF-1 activates both its downstream signaling pathways, the MEK/ERK and PI3K/Akt pathways in the cochlea, which causes more efficient effects on HC protection than activation of a single signal cascade. IGF-1 protects HCs from various injuries to the inner ear, including noise exposure, ischemia, and aminoglycoside treatment. A clinical trial of IGF-1 treatment on human SSHL as well as in vivo animal experiments has confirmed its efficacy against HC injuries.

 

Identification of axon growth promoters in the secretome of the deer antler velvet.
Pita-Thomas W1, Barroso-García G2, Moral V2, Hackett AR3, Cavalli V4, Nieto-Diaz M5.
Author information
1
Molecular Neuroprotection Group, Experimental Neurology Unit, Hospital Nacional de Paraplejicos (SESCAM), Toledo, Spain; Department of Neuroscience, Washington University School of Medicine, Saint Louis, MO, USA.
2
Proteomics Core, Hospital Nacional de Paraplejicos (SESCAM), Toledo, Spain.
3
The Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami School of Medicine, Miami, USA.
4
Department of Neuroscience, Washington University School of Medicine, Saint Louis, MO, USA.
5
Molecular Neuroprotection Group, Experimental Neurology Unit, Hospital Nacional de Paraplejicos (SESCAM), Toledo, Spain. Electronic address: mnietodiaz@gmail.com.
Abstract
Every spring, deer cast their old antlers and initiate a regeneration process, which yields a new set of antlers of up to 1m in length. Over the course of three months, branches of the trigeminal nerve, originating from the frontal skull, innervate velvet, a modified skin that covers the regenerating antler. The rate of growth of these axons reaches up to 2cm per day making them the fastest regenerating axons in adult mammals. Here, we aim to identify the factors secreted by velvet that promote such high speed axon growth. Our experiments with cultures of adult rat trigeminal neurons demonstrate that conditioned medium harvested from velvet organotypic cultures has greater axon growth-promoting properties than a medium conditioned by normal skin. The axon growth-promoting effects of velvet act synergistically with the extracellular matrix (ECM) protein laminin, a component of the basal lamina present in the deer antler. Our proteomic analyses identified several axon growth promoters in the velvet-conditioned medium (VCM), including soluble proteins such as nerve growth factor (NGF) and apolipoprotein A-1, as well as matrix extracellular proteins, such as periostin and SPARC. Additional in vitro analyses allowed us to determine that a synergic relationship between periostin and NGF may contribute to neurite growth-promoting effects of velvet secretome. A combinatorial approach using these factors may promote regeneration at high speeds in patients with peripheral neuropathies.

 

Detection of human insulin-like growth factor-1 in deer antler velvet supplements.

https://www.ncbi.nlm.nih.gov/m/pubmed/23996390/


Insulin-Like Growth Factor Signaling Regulates the Timing of Sensory Cell Differentiation in the Mouse Cochlea

http://www.jneurosci.org/content/31/49/18104.long

 

Insulin-like Growth Factor 1 in relation to future hearing impairment


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482884/

Conclusions and implications
In people at the lower end of the older-aged spectrum (aged 50 to 60 years), higher levels of IGF-1 were associated with lower odds of hearing impairment and most particularly in participants free of hearing impairment at baseline, where a moderately high effect size was found. This is the first population-based study to show a linear relationship between IGF-1 and hearing impairment. We found significant 14% decrease odds for a relatively small increase in IGF-1 (5nmol/L). The ‘J’-shaped relationship observed at older ages may partly reflect the association of IGF-1 with vascular health. With currently no existing cure for hearing loss, identification of relevant risk factors remains a priority. More observational studies are needed to confirm our results before considering the possibility of testing low-dose supplementation in IGF-1 for long-term prevention of hearing impairment.


The Role of Insulin-Like Growth Factor 1 in the Progression of Age-Related Hearing Loss

https://www.frontiersin.org/articles/10.3389/fnagi.2017.00411/full

In this context, IGF-1 emerges as a potential protector of the inner ear. Studies in animal models have shown that local application of recombinant human IGF-1 (rhIGF-I) protects the cochlea from functional and histologic losses induced by aminoglycoside ototoxicity and noise exposure (Iwai et al., 2006; Yamahara et al., 2015). IGF-1 rescued hair cells from apoptosis by downregulating pro-apoptotic gene expression and regulating glucose transporters (Yamahara et al., 2015). Similarly, IGF-1 and substance P protect vestibular hair cells against neomycin ototoxicity (Yoshida et al., 2015). Recombinant IGF-1 therapy has been approved to increase linear growth, and therefore height, in humans, spurring an increasing interest in the potential use of IGF-1 for the treatment of hearing loss (Yamahara et al., 2015). IGF-1 potential has been studied in patients with sudden sensorineural hearing loss who were resistant to treatment with systemic glucocorticoids (Nakagawa et al., 2012, 2014). A recent study with 120 patients concluded that treatment with topical IGF-1 therapy had significant effects on hearing recovery depending on their age (< 60 years) and early initiation of salvage treatment (Nakagawa et al., 2016). Prolonged activation of IGF1R by treatment with IGF-1 or analogs might have undesired secondary effects, thus their potential use to delay aging maybe limited. However available data highlight the interest of exploring IGF-1 downstream targets as drug candidates for ARHL.

 

Identification of axon growth promoters in the secretome of the deer antler velvet.
https://www.ncbi.nlm.nih.gov/pubmed/27818162

Every spring, deer cast their old antlers and initiate a regeneration process, which yields a new set of antlers of up to 1m in length. Over the course of three months, branches of the trigeminal nerve, originating from the frontal skull, innervate velvet, a modified skin that covers the regenerating antler. The rate of growth of these axons reaches up to 2cm per day making them the fastest regenerating axons in adult mammals. Here, we aim to identify the factors secreted by velvet that promote such high speed axon growth. Our experiments with cultures of adult rat trigeminal neurons demonstrate that conditioned medium harvested from velvet organotypic cultures has greater axon growth-promoting properties than a medium conditioned by normal skin. The axon growth-promoting effects of velvet act synergistically with the extracellular matrix (ECM) protein laminin, a component of the basal lamina present in the deer antler. Our proteomic analyses identified several axon growth promoters in the velvet-conditioned medium (VCM), including soluble proteins such as nerve growth factor (NGF) and apolipoprotein A-1, as well as matrix extracellular proteins, such as periostin and SPARC. Additional in vitro analyses allowed us to determine that a synergic relationship between periostin and NGF may contribute to neurite growth-promoting effects of velvet secretome. A combinatorial approach using these factors may promote regeneration at high speeds in patients with peripheral neuropathies.

Factors promoting axon growth in the deer antler
https://www.researchgate.net/publication/263034314_Factors_promoting_axon_growth_in_the_deer_antler

During their annual regeneration, antlers are innervated by trigeminal sensory axons growing at the highest rate recorded for any adult mammal. Previous analyses established the presence in the antler of nerve growth factor and neurotrophin 3 neurotrophins, which may underlie this rapid nerve growth. We are currently exploring the expression of other molecules that may be involved in such growth (axon growth promoters) combining several gene-expression techniques. Preliminary results indicate the expression of different growth promoters in the antler velvet, five of them not previously described in deer. The expression of these molecules as well as others described in the literature suggests that antler velvet promotes axon growth. However, most promoters expressed in the velvet are also present in unmodified deer skin. Thus, it must be asked why axons grow so fast in the antler? To answer that question, we developed a series of in vitro experiments using sensory neurons from adult and embryo rodents. These studies suggested that soluble proteins secreted by the velvet strongly promote neurite outgrowth. Using specific blocking antibodies, we demonstrated that nerve growth factor is partially responsible for these effects although other yet unidentified proteins seem also to be involved. The studies also showed that neither endocrine serum factors nor antler tissue substrate stimulate neurite outgrowth, although deep velvet layers cause neurite outgrowth orientation.
(PDF) Factors promoting axon growth in the deer antler. Available from: https://www.researchgate.net/publication/263034314_Factors_promoting_axon_growth_in_the_deer_antler [accessed Oct 11 2018].


[neurotrophin 3 is being discussed in other thread]

 

Today I started with this.
Dosage: 4 Tabs/day.

 

I hope this thing works for you and subsequently us.

 

I don't expect a miracle. My hopes are with Celastrol rather.

 

Not to be pessimistic but I dont think we can take enough of this stuff safely to do what we want it to do. That's why curcumin excited me so much, because you take mega doses.

 

How big is your Curcumin dose?